4 research outputs found
Oxidation of tertiary amine-derivatized surfaces to control protein adhesion
Selective oxidation of omega-tertiary amine self-assembled thiol monolayers to tertiary amine N-oxides is shown to transform the adhesion of model proteins lysozyme and fibrinogen upon them. Efficient preparation of both secondary and tertiary linker amides as judged by X-ray photoelectron spectroscopy (XPS) and water droplet contact angle was achieved with an improved amide bond formation on gold quartz crystal microbalance (QCM) sensors using 2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl hexafluorophosphate methanaminium uronium (HATU). Oxidation with hydrogen peroxide was similarly assessed, and adhesion of lysozyme and fibrinogen from phosphate buffered saline was then assayed by QCM and imaged by AFM. Tertiary amine-functionalized sensors adsorbed multilayers of aggregated lysozyme, whereas tertiary amine N-oxides and triethylene glycol-terminated monolayers are consistent with small protein aggregates. The surface containing a dimethylamine N-oxide headgroup and ethyl secondary amide linker showed the largest difference in adsorption of both proteins. Oxidation of tertiary amine decorated surfaces therefore holds the potential for selective deposition of proteins and cells through masking and other patterning techniques
Using bio-adhesive and bio-inert surfaces to maximize biogas production and influence microbial growth in anaerobic digesters
The optimisation of biogas digesters is crucial for further development of sustainable energy sources. In this research, an integrative approach was taken to understanding how this problem can be addressed including: seeking a better understanding of protein – surface interactions on the molecular level; larger scale experiments to screen the best materials for use in laboratory scale anaerobic digesters to influence microbial growth and biofilm development as well as analysis of farm-scale data using the ADM1 multiparameter model.
The laboratory-scale experiments were undertaken to develop surfaces suitable for studying microbial immobilization. This work, currently using tertiary amines, amine oxides and comparator oligoethylene glycol studies the adsorption of two classic model proteins: lysozyme and fibrinogen using quartz crystal microbalance methods and represents important steps for selecting and exploring surface – protein interactions. The data showed that tertiary amine oxides are more resistant to nonspecific protein adsorption than the corresponding tertiary amines.
Heat modified polyurethane foam was used to explore biofilm and planktonic phase microbial populations in a fixed film biogas reactor.. After four weeks the foam was analysed by ‘next generation’ 454-sequencing to identify the influence of the supporting materials on microbial population residing in anaerobic digesters. The results revealed that Spirochaetes, Methanobacterium and Methanocorpusculum associated themselves with heat modified polyurethane foams.
Finally, data from a farm-scale anaerobic digester (volatile fatty acid concentration, temperature and pH) have been gathered and entered into the ADM1 model, developed by the mathematical modelling group from Lund University, to mimic the behaviour of a laboratory scale 1.5 l reactor and identify improved conditions for methanogens stability.
The combination of approaches described above will allow the identification of which parameters will enhance the operation of anaerobic digesters and has identified surfaces that promote adhesion of particular Bacteria and Archaea in order to increase biogas production
Protein-adhesive and protein-resistant functionalized silicon surfaces
A series of new ω-alkenyl tertiary amine N-oxides is prepared in solution and immobilized on hydrofluoric acid-etched silicon {111} wafers. These monolayers are characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy (AFM) and tested for their resistance to non-specific protein adhesion with two model proteins, lysozyme and fibrinogen. The use of silicon substrates is found to give good quality tertiary amine N-oxidemonolayers and these new surfaces are found to be significantly better at preventing non-specific protein adhesion than their parent amines as judged by AFM imaging
Psychology in Administration: A Research Orientation, par T.W. Costello et S.S. Zalkin, Prentice-Hall, Inc., Englewood Cliffs, N.J., 1963. 500 pages.
Selective
oxidation of ω-tertiary amine self-assembled thiol
monolayers to tertiary amine <i>N</i>-oxides is shown to
transform the adhesion of model proteins lysozyme and fibrinogen upon
them. Efficient preparation of both secondary and tertiary linker
amides as judged by X-ray photoelectron spectroscopy (XPS) and water
droplet contact angle was achieved with an improved amide bond formation
on gold quartz crystal microbalance (QCM) sensors using 2-(1<i>H</i>-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl hexafluorophosphate
methanaminium uronium (HATU). Oxidation with hydrogen peroxide was
similarly assessed, and adhesion of lysozyme and fibrinogen from phosphate
buffered saline was then assayed by QCM and imaged by AFM. Tertiary
amine-functionalized sensors adsorbed multilayers of aggregated lysozyme,
whereas tertiary amine <i>N</i>-oxides and triethylene glycol-terminated
monolayers are consistent with small protein aggregates. The surface
containing a dimethylamine <i>N</i>-oxide headgroup and
ethyl secondary amide linker showed the largest difference in adsorption
of both proteins. Oxidation of tertiary amine decorated surfaces therefore
holds the potential for selective deposition of proteins and cells
through masking and other patterning techniques